Turnip mosaic virus ( tumv ) and cucumber mosaic virus ( cmv ) are two worldwide spread plant rna viruses , whose damages to host plants are utmost serious 蕪菁花葉病毒( turnipmosaicvirus , tumv )和黃瓜花葉病毒( cucumbermosaicvirus , cmv )是兩種世界范圍內(nèi)分布極為廣泛、危害極為嚴(yán)重的植物病毒。
Upon inoculation of cucumber mosaic virus ( cmv ) , the symptom development in the transgenic plants was delayed , one week later than that of the non - transgenic control , and the symptom on the transgenic plants were much milder Pcr反應(yīng)以及southern雜交實(shí)驗(yàn)表明,外源基因片段已經(jīng)插入到番茄的染色體中。用黃瓜花葉病毒cmv接種轉(zhuǎn)基因番茄植株,其癥狀出現(xiàn)的時(shí)間比非轉(zhuǎn)基因番茄對(duì)照要延遲7天左右。
Dsmv is proved as the predominating virus - pathogen on aroid plants from zhejiang province and other regions in china . cdna of dsmv rna 3 " end partial sequence and subgenomic rna promoter region of cucumber mosaic virus ( cmv ) rna3 were used as probes for detection of dsmv and cmv respectively . total rna extracted from field samples were used for rna dot - hybridization 用侵染馬蹄蓮的dsmv3末端序列和黃瓜花葉病毒( cmv )的亞基因組啟動(dòng)子區(qū)互補(bǔ)dna序列為標(biāo)記探針,對(duì)自然感病的天南星科植物進(jìn)行rna斑點(diǎn)雜交,并結(jié)合雙鏈rna分析、病毒提純和形態(tài)學(xué)觀察,對(duì)杭州等地16屬天南星科植物的81個(gè)樣品進(jìn)行了病毒鑒定。